“AgriPheno論壇”（8月20日）：Applications of Automated DNA Mass Spectrometry in Agribiotech

報告題目：Applications of Automated DNA Mass Spectrometry in Agribiotech
報 告 人：Dr. Darryl Irwin, Director–Applications & Technology, Asia Pacific, Sequenom
報告時間：2014年8月20日14:30
報告地點：上海市浦東新區(qū)沔北路185號孫橋現(xiàn)代農(nóng)業(yè)園C9-1 AgriPheno平臺2樓會議室
聯(lián) 系 人：魏淵源(yywei@zealquest.com)
報告摘要：

Sequenom’s automated DNA analysis platform has recently been broadly used in a variety of species important in agriculture and livestock. A single MassARRAY system can be used for the analysis of DNA sequence variations like SNPs, gene expression, DNA methylation, copy number variation and certain DNA sequencing applications. The sensitivity is 100 to 1,000 times higher than real time PCR and the quantification is an order of magnitude better than real time PCR. But perhaps the strongest feature of the technology is the ease and flexibility with which new problem-specific applications can be developed and validated. This is especially important for agribiotech where so many diverse species are of interest. For example, with commercial livestock and crops, panels of 10s to 100s of SNPs can be rapidly developed that are extremely powerful for individual identification, breeding, and feedlot/nutrient management. The increased use of high content discovery technologies such as 2nd Generation Sequencing and microarrays in agricultural research has resulted in a clear need for validation of discovered markers. With plants, ploidies higher than 2, mean that essentially every locus must be treated as a potential copy number variant, and mass spectrometry offers a facile way to quantify such variants in a cost-effective multiplexed manner. DNA mass spectrometry also shows great promise in the automated identification and typing of micro-organisms, and thus might be very useful in the study of a wide variety of plant and animal infectious diseases. This seminar will review the basics of the technology and then illustrate a number of current case studies of the above applications.

關(guān)于“AgriPheno論壇”
“AgriPheno論壇”是由高通量植物基因型-表型-育種平臺AgriPheno推出的一個學(xué)術(shù)交流活動平臺，旨在促進(jìn)國內(nèi)外相關(guān)學(xué)者、育種家、發(fā)明家和應(yīng)用科學(xué)家與AgriPheno平臺和上海的農(nóng)業(yè)科技人員之間的交流、互動。該論壇不定期于AgriPheno平臺（上海市浦東新區(qū)沔北路185號孫橋現(xiàn)代農(nóng)業(yè)園C9-1）舉行，每次會提前通過網(wǎng)站、微信等渠道發(fā)布通知。我們誠摯邀請相關(guān)學(xué)者、育種家、發(fā)明家和應(yīng)用科學(xué)家在訪問AgriPheno平臺時通過講座報告的形式參與該論壇；我們熱誠歡迎相關(guān)科研人員和研究生參與論壇的交流活動。